GAA for intestinal function
The current study is designed to investigate dietary guanidinoacetic acid (GAA) supplementation on the growth performance, intestinal histomorphology, and jejunum mucosal barrier function of broilers that subjected to chronic heat stress (HS). A total of 192 male broilers (28-d-old) were randomly allocated to four groups. A chronic HS model (at a temperature of 32 °C and 50 to 60% relative humidity for 24 h daily) was applied in the experiment. Normal control (NC, ad libitum feeding, 22°C), heat stress group (HS, ad libitum feeding, 32°C), pair-fed group (PF, received food equivalent to that consumed by the HS group on the previous day, 22°C), guanidinoacetic acid group (HG, ad libitum feeding, supplementing the basal diet with 0.6 g/kg GAA, 32°C). The experiment was lasted from 28 to 35 and 28 to 42 of age of broilers. Our results showed that broilers subjected to HS had lower average daily feed intake and average daily gain (P < 0.05), higher feed-to-gain ratio and relative length of the small intestine (P < 0.05), as well as lower relative weight and weight per unit length of the small intestine (P < 0.05). HS damaged the small intestinal histomorphology by decreasing the small intestinal VH and the VH/CD (P < 0.05). Compared with the HS group, supplementation with 0.6 g/kg GAA increased jejunal VH and VH/CD (P < 0.05), but decreased relative weight and relative length of the small intestine (P < 0.05). Moreover, in comparison with NC, HS elevated intestinal permeability (D-Lactic acid concentration and diamine oxidase activity) and mRNA expression levels of interleukin-1β, interleukin-6, and tumor necrosis factor-α (P < 0.05), reduced jejunal mucus thickness, number of goblet cells, IgA + cell density, and mucin2 mRNA expression level of broilers (P < 0.05). Compared with the HS group, dietary GAA elevated jejunal mucus thickness, goblet cell number and IgA + cell density (P < 0.05), and up-regulated jejunal mRNA expression of interleukin-1β and tumor necrosis factor-α (P < 0.05). In conclusion, heat stress impaired growth performance, and the intestinal mucosal barrier function of broilers. Dietary supplementation with 0.6 g/kg GAA alleviated heat stress-induced histomorphology changes of small intestine and jejunal mucosal barrier dysfunction.
Peng XY, Xing T, Li JL, Zhang L, Jiang Y, Gao F. Guanidinoacetic acid supplementation improves intestinal morphology, mucosal barrier function of broilers subjected to chronic heat stress. J Anim Sci. 2022 Oct 25:skac355. doi: 10.1093/jas/skac355.