We developed and evaluated a colorimetric method for enzymic determination of guanidinoacetic acid (GAA) in urine. Endogenous urinary urea was first eliminated by urease (EC 3.5.1.5), and the added urease was then removed from the sample by centrifugal ultrafiltration. GAA in the ultrafiltrate was subsequently hydrolyzed by guanidinoacetate amidinohydrolase (EC 3.5.3.2) to glycine and urea. […]
Read MoreA new enzymic determination of GAA in urine
- March 16, 1987
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