Creatine is a popular athletic supplement that has also been shown to improve cognitive performance upon sleep deprivation. However, it is rapidly cleared from the gastrointestinal tract a few hours after consumption. Toward providing a persistent creatine dose, we engineered the human probiotic Escherichia coli Nissle (EcN) to produce guanidinoacetic acid (GAA), which is converted […]
Read More
Guanidinoacetic acid (GAA) has been used in ruminant feeding, but it is still unclear whether the exogenous addition of methyl donors, such as methionine (Met), can enhance the effects of GAA. This study investigated the effects of dietary GAA alone or combined with Met on beef cattle growth performance and explored the underlying mechanisms via […]
Read More
To establish an indirect competitive ELISA (ci-ELISA) for the detection of guanidino acetic acid (GAA) residues in animal feed, in this study, GAA was coupled to carrier proteins via the active ester method to obtain an anti-GAA complete antigen (GAA-BSA) and a detection antigen (GAA-OVA). BALB/c mice were immunized with GAA-BSA, after which anti-GAA monoclonal […]
Read More
Altered gut microbiota‒metabolite interactions may result in intestinal inflammation severity variation in Crohn’s disease (CD). Magnetic resonance enterography (MRE) advances anti-inflammatory strategy development. We aimed to identify inflammation-related multiomics factors and MRE interactions for CD management, analyzing 425 CD patients and 42 healthy controls undergoing MRE, ileocolonoscopy, and fecal/blood sampling (microbiota/metabolite analyses), with intestinal inflammation […]
Read More
This study aimed to investigate the effects of GAA supplementation in diets differing in ME levels on productive performance, egg quality, blood parameters, yolk fatty acid profiles, hepatic expression of genes related to lipid metabolism, gut morphology, and nutrient digestibility in laying hens during their post-peak production phase. Over a 12-week period (52-64 weeks of […]
Read More
The increasing incidence of obesity has been associated with various diseases, including inflammatory bowel disease. Guanidinoacetic acid (GAA) regulated various crucial physiological processes. The present study aimed to determine the action of dietary GAA on high-fat diet (HFD)-induced colitis in middle-aged mice. Mice (8 months old) were fed chow or HFD with or without GAA […]
Read More
Guanidinoacetic acid (GAA) is immediate substrate for biosynthesis of creatine (CREA). Aside from improving energy utilisation, GAA possesses several features which further enhance its value as a feed additive for poultry. The feed supplement has good thermal stabi lity, which allows it to be safely used in pelleted diets. GAA has high bioavailability, is cost-effective, […]
Read More
Burns are a common and serious health issue, with severe burn-induced acute kidney injury (AKI) being a major factor contributing to poor recovery and increased mortality in patients. Theabrownins (TBs), bioactive compounds formed during tea leaf fermentation, have shown promising effects on reducing inflammation, combating oxidative stress, and enhancing metabolic function. However, the roles and […]
Read More
The aim of this study was to investigate the effects of including guanidinoacetic acid (GAA) in the diet of Jersey cows during their first lactation, evaluating the effects on the ruminal environment, productivity, milk composition and quality, and hematological variables. The study used 18 Jersey animals in their first lactation with 230 days of lactation. […]
Read More
The effectiveness of guanidinoacetic acid (GAA) in reduced protein (RP) diets on performance and gut health of broilers under heat stress is largely unknown. A 35-d experiment was conducted using four dietary treatments: a standard protein diet (SP, 22.1 and 20.7% CP in grower and finisher), a RP diet (20.1 and 18.7% in grower and […]
Read More